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Peroxiredoxin 6: a bifunctional enzyme with glutathione peroxidase and phospholipase A2 activities

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Activities of NADH-ubiquinone-oxidoreductase (Complex I), succinate-cytochrome c reductase (Complex II/III), and cytochrome c oxidase (Complex IV) were measured by the methods of Birch-Machin and Turnbull ( Assessing protein expression by SDS-PAGE and western blotting Cellular fractions (mitochondria, cytosol, 3050 g protein) from the liver and kidneys of control Wistar and diabetic GK rats were electrophoretically separated by 7.5%12% SDS-PAGE ( Statistical analysis Values are expressed as mean SD of three-five biological replicates

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73 The proteins or the receptors must be salvaged back to the exterior membrane of cells